Gel electrophoresis of Spherical Colloidal Particles
Date Issued
2011
Date
2011
Author(s)
Tsai, Peter
Abstract
Gel-electrophoresis of spherical colloidal particles is investigated theoretically in this thesis. Both the short range steric effect (due to the direct contact friction between the solid obstacles and the migrating particles) and the long range hydrodynamic effect (due to the hydrodynamic force of the gel exerted upon the liquid suspension) are considered with the particle mobility calculated as the product of the predictions from these two approaches separately. Brinkman model is assumed to describe the fluid flow in the polymer gels. A pseudo-spectral method based on Chebyshev polynomials is adopted to solve the resulting general electrokinetic equations.
We find, among other things, that the deformation of an ion clouds surrounding the particles due to the convection flow within the polymer gels deters the particle motion significantly, as an induced electric field opposite to the applied electric field is generated, an effect referred to as the polarization effect here. The higher the surface potential of the particle is, the more significant the reduction of mobility due to this polarization effect. Local extrema are observed in the mobility profiles with varying double layer thickness around
Subjects
gel-electrophoresis
effective medium theory
polarization effect
double layer overlapping
colloidal suspension
Brinkman fluid
boundary effect
Type
thesis
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