Magnetic nanoparticle labeling of cultured cancer cell line without transfection agent
Journal
Biomedical Engineering - Applications, Basis and Communications
Journal Volume
20
Journal Issue
4
Pages
259-265
Date Issued
2008
Author(s)
Abstract
Magnetic nanoparticle (MNP) labeling of stem cell has been proved its efficacy for cell trafficking. Most of the labeling technique requires mixture of iron oxide nanoparticles and transfection agent. Stem cells with ionic MNP without the aid of transfection agent were labeled previously. The possibility of high efficiency labeling of cultured cancer cell, HeLa cell, by using ionic MNP is proposed. The labeled cell morphology was observed and the intracellular iron content was determined by spectrophotometry. The cell character change was evaluated by flow cytometry where front scattering count and side scattering count (SSC) were recorded. The imaging ability of the labeling method was determined by T2 weighted magnetic resonance (MR) imaging. Labeled MNPs were accumulated at cytoplasm is observed and the iron content of labeled cell could reach 27 pg/cell. There is no cell diameter change but the cell granularity increased according to SSC data from flow cytometry. Under clinical 1.5T MR imaging, we could detect labeled cells easily were detected at the cell number of 1 × 105. It is concluded that labeling of cancer cell with ionic MNPs without transfection agent is an efficient labeling method which will provide non-invasive imaging method for monitoring cancer behavior. ? 2008 National Taiwan University.
SDGs
Other Subjects
Cell culture; Cytology; Diseases; Flow cytometry; Image processing; Iron; Iron oxides; Magnetic resonance imaging; Magnetism; Nanomagnetics; Nanoparticles; Stem cells; Cancer cell lines; HeLa cell; Intracellular iron; Iron oxide nanoparticle; Labeling techniques; Magnetic nano-particles; Non-invasive imaging; Transfection agents; Molecular biology; iron; nanoparticle; article; cancer cell culture; cell count; cell structure; cytoplasm; female; flow cytometry; HeLa cell; human; human cell; imaging; neoplasm; nuclear magnetic resonance imaging; spectrophotometry
Type
journal article
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