Anti-Cd45 Isoform Antibodies Enhance Phagocytosis and Gene Expression of Il-8 and Tnf-Alpha in Human Neutrophils by Differential Suppression on Protein Tyrosine Phosphorylation and P56(Lck) Tyrosine Kinase
Resource
CLINICAL AND EXPERIMENTAL IMMUNOLOGY v.129 n.1 pp.78-85
Journal
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
Journal Volume
v.129
Journal Issue
n.1
Pages
78-85
Date Issued
2002
Date
2002
Author(s)
YU, CHIA-LI
Abstract
To determine the biological functions of membrane expressed CD45 isoforms on polymorphonuclear neutrophils (PMN), the monoclonal IgG F(ab')(2) antibody against CD45, CD45RA or CD 45RO was used as surrogate ligand for binding with these molecules on PMN. We found 99.5 +/- 3.2%, 42.3 +/- 5.8% and 96.7 +/- 2.6% PMN expressed CD45, CD45RA and CD45RO molecules on the cell surface, respectively. The interaction of CD45, CD45RA or CD45RO with its specific antibody on PMN enhanced phagocytosis markedly (34-83% increase), mainly via increased expression of complement receptor type 3 ( CR3, CD11b) on the cells. The production of IL-8 by PMN was also increased significantly after binding with antibodies (anti -CD45 > anti-CD45RO > anti-CD45RA). Anti-CD45RA and anti-CD 45RO, but not anti-CD45, enhanced TNF-alpha mRNA expression and decreased protein tyrosine phosphorylation of PMN. However, only anti-CD45RO suppressed Src family protein tyrosine kinase p56(lck) expression in the cells. These results suggest that the cross-linking of CD45 isoforms by their specific antibodies stimulated different PMN activities by differential suppression on protein tyrosine phosphorylation and Src family tyrosine kinase p56(lck) .
Subjects
leucocyte-common antigen
PMN phagocytosis
proinflammatory cytokines
protein tyrosine kinase p56lck
LEUKOCYTE-COMMON ANTIGEN
IFN-GAMMA PRODUCTION