Immune characterization of peripheral blood mononuclear cells of the dogs recovered from canine transmissible venereal tumor inoculation
Date Issued
2012
Date
2012
Author(s)
Lin, Hsiu-Chen
Abstract
Canine transmissible venereal tumor (CTVT) is a tumor which can be transmitted naturally through mucosa contact between dogs. After CTVT cells were inoculated to healthy dogs, it will grow rapidly and then regress spontaneously. Therefore, it is a good model to investigate the interactions between tumor and host immune system. Previous studies have shown that CTVT cells can not survive in recovered tumor-inoculated dogs. Thus, the present study was designed to investigate the immunity changes of peripheral blood mononuclear cells (PBMCs) from “uninoculated” or “CTVT inoculated and recovered” canines after cocultured with CTVT cells in vitro to infer the possible mechanisms. The analysis of lymphocyte phenotypes showed that CD3, CD4, CD8, or CD21 expression percentage had no significant difference between these two groups. After cocultured of PBMCs from these two sources with P phase CTVT cells, cell proliferation, IFN-γ secretion, and cell cytotoxicity were examined. The degrees of mRNA expression of proinflammatory cytokines (TNF-α, IL-1β, IL-6, TGF-β), Th1 cytokines (IL-2, IFN-γ), Th2 cytokines (IL-4, IL-10) and cytotoxic proteins (Granzyme B, Perforin) were assayed as well. The results indicated that no significant difference were observed in most criteria tested except that cytokines gene expression of IL-1β and IL-6 in uninoculated canine was higher than in CTVT recovered canines (p <0.05). IL-1β and IL-6 are proinflammatory cytokines which has a strong pro-tumorigenic activity to promote tumor cell proliferation and survival and even can cause tumor angiogenesis and metastasis. It is thus speculated that secondary inoculated CTVT cells can not survive may be due to the insufficient production of IL-1β and IL-6. Further investigation is requied to explore the exact roles of these cytokines in CTVT growth.
Subjects
CTVT
PBMC
cytokine
Type
thesis
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