Cytoskeleton and Heart Failure
Date Issued
2000-07-31
Date
2000-07-31
Author(s)
朱樹勳
DOI
892314B002190
Abstract
Cardiovascular diseases, like dilated cardiomyopathy (DCM), are usually very complex
and the elucidation of pathogenic mechanisms is very difficult. Dilated cardiomyopathy
(DCM) is a severe heart disease leading to heart insufficiency and most heart transplantations
are indicated by DCM. We employed proteomics methods to analyze myocardial proteins
derived from DCM myocardium, particularly myofibril proteins. We successfully isolated
myofibril proteins using differential extraction method and then resolved them on a 2D gel
electrophoresis system. The identities of proteins are verified using liquid chromatographytandem
mass spectrometry and specific modifications on these proteins can also be identified.
Using myosin regulatory light chain 2 (mlc-2) as an example, we demonstrated that how
modification is mapped and quantitatively determined using selected ion tracing approach.
One patient in our reseach has 3.7% phosphorylation at Ser-14, while the other two both have
a phosphorylation percentage of ~16%. The significance of this difference will be validated as
more samples are analyzed.
Subjects
dilated cardiomyopathy
proteomics
tandem mass spectrometry
twodimensional
gel electrophoresis
gel electrophoresis
SDGs
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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