Hepatoprotection of Gentiana scabra Extract and Polyphenols in Liver of Carbon Tetrachloride- intoxicated mice
Journal
Journal of Environmental Pathology, Toxicology and Oncology
Journal Volume
30
Journal Volume
30
Journal Issue
3
Journal Issue
3
Pages
179-187
Start Page
179
End Page
187
ISSN
07318898
Date Issued
2011
Author(s)
Abstract
In traditional Chinese medicine, the rhizomes of Gentiana scabra are used to treat hepatitis, stomatitis, and inflammatory diseases. This study aimed to examine the protective effects of G. scabra aqueous extract (GS) and polyphenols in liver of CCl4-intoxicated mice. Antioxidant activities of GS were also evaluated. Results showed that GS exhibited anti-lipid peroxidation, 2,2-diphenyl-1-picrylhydrazyl, and superoxide radical scavenging activities with IC50 values of 45.84, 183.38, and 56.25 μg/mL, respectively. High-performance liquid chromatography analysis revealed that the major polyphenolic constituents of GS were kaempferol, ellagic acid, and quercetin. Daily oral administration of 500 mg/kg GS (GS500) and 1000 mg/kg GS (GS1000) significantly prevented the elevation of glutamic pyruvic transaminase and thiobarbituric acid reactive substances levels, while enhancing the levels of antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) and serum total antioxidant activity in mice with hepatoxicity. At 1000 mg/kg, GS was as effective as 100 mg/kg silymarin in reducing oxidative stress and preventing liver injury. Histopathological studies further confirmed the hepatoprotective activity of GS. Taken together, these results show that the antioxidant activities and polyphenolic compounds (kaempferol, ellagic acid, and quercetin) of GS may have contributed to its hepatoprotective activity in CCl4-intoxicated mice, and its mechanism of action could be mediated through the reduction of oxidative stress in liver tissue.
Subjects
Antioxidant
Gentiana scabra
Hepatoprotective
Polyphenols
SDGs
Other Subjects
1,1 diphenyl 2 picrylhydrazyl; alanine aminotransferase; antioxidant; carbon tetrachloride; catalase; ellagic acid; Gentiana scabra extract; glutathione peroxidase; kaempferol; plant extract; polyphenol; quercetin; scavenger; silymarin; superoxide; superoxide dismutase; thiobarbituric acid reactive substance; unclassified drug; animal experiment; animal model; antioxidant activity; article; controlled study; drug activity; drug effect; enzyme activity; enzyme blood level; gentian; Gentiana scabra; high performance liquid chromatography; histopathology; IC 50; intoxication; lipid peroxidation; liver injury; liver protection; liver toxicity; male; mouse; nonhuman; oxidative stress; priority journal
Publisher
Begell House Inc.
Type
journal article