Using In Vivo Confocal Neuroimaging to observe rat retinal wave
Date Issued
2012
Date
2012
Author(s)
Huang, Bo-Jyun
Abstract
It has been shown that the spontaneous activity of neurons, called retinal wave, in immature retina of vertebrate will influence retina growth and connection between CNS.The phenomenon using electrophysiological measurment in vivo or in vitro and observation of optical microscope from cultured retina were successfully detected, but those technology were invasive.Because of the transparency of the eyes, retina is the only part of central nervous system (CNS) that can be investigated noninvasively by optical imaging. In this report, we applied in vivo confocal neuroimaging (ICON) to detect the electrical activities of neurons in postnatal rat retina. ICON permits repeated observation of rats, preventing cell damage as well as rat sacrifice. In addition, ICON has the potential to provide subcellular resolution at fundus, so that the retinal neuron activities can be studied in more detail.
Our experiments can’t approve the spatial property of the retinal waves, but confirmed that the neural activity within living organisms. The same rat can be repeatedly observed for a long time, thus provideing a possibility of detcting neural activity in vivo. We can investigate the relationship between the effects triggered by patterned spontaneous activity on the connectivity between different neuron populations (happening from days to weeks after birth) and the plasticity of the synapses between neurons as induced by action potential (occurring in milliseconds to seconds).
Subjects
retinal wave
confocal
Type
thesis
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