A new approach to deciphering pathways of enterovirus 71-infected cells: An integration of microarray data, gene ontology, and pathway database
Journal
Biomedical Engineering - Applications, Basis and Communications
Journal Volume
18
Journal Issue
6
Pages
337-342
Date Issued
2006
Author(s)
Abstract
The enterovirus 71 infection is associated with severe neurological disease in several clinical researches; however, the detailed gene network mechanisms of enterovirus 71-infected cells remain unclear at present. We present a new approach integrating microarray expression data, KEGG database, gene ontology (GO), and OMIM information for efficiently deciphering pathways of enterovirus 71-infected cells. This approach includes the following steps: (1) profiling the significant gene-gene interaction through pathway database, (2) utilizing Fisher's exact test to analyze pathway information and to rank the first ten significant pathways, (3) annotating functions of genes in the pathways through gene ontology, (4) investigating related genes and perhaps concern diseases by referring to OMIM information. Our findings illustrate at least three possible pathways in enterovirus 71-infected human neural SF268 cells: Jak-STAT signaling: cell cycle and apoptosis. Furthermore, we show that some genes are associated with neural development and neural apoptosis, such as c-Myc, BAX, NGF, and CPP32. These would be useful for profiling disease mechanisms and host response to virus in future research.
Subjects
Enterovirus 71; Gene ontology; Miocroarray; Pathway
SDGs
Other Subjects
Data reduction; Database systems; Genes; MIM devices; Ontology; Viruses; Enterovirus 71; Gene ontology (GO); KEGG database; Miocroarrays; Cells; Janus kinase; STAT protein; apoptosis; article; cell cycle; controlled study; data base; Enterovirus 71; Enterovirus infection; Fisher exact test; gene expression; human; human cell; microarray analysis; neurologic disease; Human enterovirus 71
Type
journal article