Microvessel density, cyclo-oxygenase 2 expression, K-ras mutation and p53 overexpression in colonic cancer
Journal
British Journal of Surgery
Journal Volume
91
Journal Issue
3
Pages
355-361
Date Issued
2004
Author(s)
Abstract
Background: Tumour angiogenesis, cyclo-oxygenase (COX) 2 expression, K-ras mutation and p53 overexpression are commonly involved in colorectal tumorigenesis, but their interrelationship and clinicopathological effects remain inconclusive. Methods: Clinicopathological data from 114 consecutive patients with primary stage III colorectal cancer were evaluated prospectively. Microvessel density (MVD) of the tumour was denned by counting the number of microvessels in hotspots, visualized by immunocytochemical staining of endothelial CD34. K-ras mutation was analysed by the restriction enzyme cleavage method. COX-2 expression and p53 overexpression were determined by immunocytochemistry. Results: Increased MVD in hotspots was significantly associated with COX-2 expression (P < 0.001), K-ras mutation (P = 0.007) and p53 overexpression (P = 0.006). COX-2 expression was not associated with either K-ras mutation or p53 overexpression. Clinicopathologically, greater MVD and COX-2 expression were significantly associated with vascular invasion of cancer cells (MVD, P = 0.027 and COX-2 expression, P = 0.006), but p53 overexpression and K-ras mutation were not. Multivariate analysis indicated that greater MVD (P = 0.002) and p53 overexpression (P = 0.016) were significant independent predictors of tumour recurrence, whereas COX-2 expression (P = 0.634) and K-ras mutation (P = 0.356) were not. Conclusion: Tumour angiogenesis may be associated with tumour metastasis and is significantly influenced by K-ras mutation, p53 overexpression and COX-2 expression in patients with colonic cancer.
SDGs
Other Subjects
CD34 antigen; cyclooxygenase 2; fluorouracil; folinic acid; K ras protein; protein p53; adult; aged; angiogenesis; article; cancer staging; carcinogenesis; clinical feature; colon cancer; female; gene mutation; gene overexpression; human; human tissue; immunocytochemistry; major clinical study; male; microvasculature; multivariate analysis; priority journal; prospective study; protein expression; restriction mapping; Adult; Aged; Carcinoembryonic Antigen; Colonic Neoplasms; Cyclooxygenase 2; Female; Genes, p53; Genes, ras; Humans; Immunohistochemistry; Isoenzymes; Male; Membrane Proteins; Microcirculation; Middle Aged; Mutation; Neoplasm Metastasis; Neovascularization, Pathologic; Prostaglandin-Endoperoxide Synthases; Tumor Suppressor Protein p53
Type
journal article