The Effect of DMSO in Drosophila Larvae Studied by Multiphoton Microscopy

Neuron science research has been more and more popular recently. The most attractive organism in this study is brain, and one of the most famous model samples is drosophila larva. Although almost whole brain structure map studied by genetic engineering has been constructed, it still only provides structural connection, but not functional connection. In order to further prove the correlation of these two connections, we use calcium dye and voltage sensitive dye as our neuron signal indicator and construct a confocal microscopy to detect it. Both of these dyes should be dissolved into dimethyl sulfoxide (DMSO) solution, thus, analyzing the influence of DMSO on our sample drosophila larva is also important. According to the principle of second harmonic microscopy (SHG), signal intensity will depend on the molecular structure of samples. We analyze the SHG signal of our sample after soaking in phosphate buffered saline (PBS) solution and DMSO solution respectively. Samples soaked in DMSO lead to the enhancement of SHG signal, and that implies there would be some structural changes in our organism samples. We also find that the performance of green fluorescence protein (GFP) of two-photon fluorescence (TPF) will vanish after DMSO soaked. This result may provide a new perspective for those who want to load dyes on drosophila larvae.