Isolation and characterization of new lactam compounds that inhibit lung and colon cancer cells from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) bran
Resource
Food and Chemical Toxicology, 46(6), 1933-1939
Journal
Food and Chemical Toxicology
Pages
1933-1939
Date Issued
2008
Date
2008
Author(s)
Lee, Ming-Yi
Lin, Huan-You
Cheng, Faiwen
Chiang, Wen-Chang
Kuo, Yueh-Hsiung
Abstract
Five active compounds that inhibit cancer cells were isolated from adlay bran (Coix lachryma-jobi L. var. ma-yuen Stapf), and their structures and activities in vitro were characterized. The ethyl acetate-soluble fraction of methanol extracts of adlay bran (ABM-EtOAc) exhibited a stronger anti-proliferative effect on human lung cancer cell A549, human colorectal carcinoma cell HT-29, and COLO 205 than other fractions by MTT (3-4,5-dimethylthiazol-2-yl-2,5-diphenyl-tetrazolium bromide) assay. Assay-guided isolation gave five lactams including three that were previously undocumented; coixspirolactam A (1), coixspirolactam B (2), and coixspirolactam C (3); one isolated from the natural plant for the first time, coixlactam (4); and one known compound, methyl dioxindole-3-acetate (5). Pure active compounds were identified by spectral analysis including IR, 1H and 13C NMR, UV-vis, MS and 2D NMR techniques. All the compounds were tested for their anti-proliferative effect on A549, HT-29 and COLO 205 cells. These compounds showed anti-cancer activities with IC50 values between 28.6 and 72.6 μg/mL. ? 2008 Elsevier Ltd. All rights reserved.
Subjects
Adlay bran; Anti-proliferative; Colorectal carcinoma; Lactam; Lung cancer
SDGs
Other Subjects
3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; acetic acid ethyl ester; Coix lachryma jobi extract; coixlactam; coixspirolactam a; coixspirolactam b; coixspirolactam c; lactam derivative; methanol; methyl dioxindole 3 acetate; plant extract; antineoplastic activity; article; bran; cancer cell; cell proliferation; Coix; coix lacryma jobi; colorectal carcinoma; controlled study; drug activity; drug isolation; drug structure; human; human cell; lung cancer; medicinal plant; nuclear magnetic resonance spectroscopy; Antineoplastic Agents, Phytogenic; Cell Proliferation; Coix; Colonic Neoplasms; HT29 Cells; Humans; Lactams; Lung Neoplasms; Magnetic Resonance Spectroscopy; Mass Spectrometry; Methanol; Solvents; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Tetrazolium Salts; Thiazoles; Coix lacryma-jobi
Type
journal article
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Isolation and characterization of new lactam compounds that inhibit lung and colon cancer cells from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) bran.pdf
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