Detection of Anti-epileptic Drug Valproic Acid by Piezoresistive Microcantilever Biosensor with Electric Field Enhanced Probing Protein Immobilization
Date Issued
2012
Date
2012
Author(s)
Chang, Kai-Fung
Abstract
Therapeutic drug monitoring is a growing issue to measure specific drugs at timed intervals in order to maintain a relatively constant concentration of the medication in the bloodstream. Recognition and quantification of bio-molecules are irreplaceable in biomedical tests and disease diagnosis. This study focus on the piezoresistive microcantilever biosensor embedded with electrodes for manipulation and enhancement of probing protein immobilization onto sensing surfaces. The electrically protein-manipulated, nanomechanics-based biosensor is featured with significant reduction of usage in probing biomaterials, low production cost and high sensistivity; in addition, real-time detection provide immediate and efficient cares for patients at home or bedside point of care.
Connecting ITO conductive film and piezoresisitive film in microcantilever, to fabricate a biosensor that is capable of manipulating electric field. Based on changes in electrical charges of the protein in different solution environments, the approach in this study enhanced the immobilization efficiency of probing protein onto the surfaces of microcantilever by applying electric field.
As expected, most charged proteins distributed in solution are effectively attracted onto the sensing gold within electric field in high voltage. Under the influence of electric field, the experiment successfully increased the effectiveness of probing protein immobilization by 3 times in which the microcantilever was used in real-time measurement of Valproic acid drug, and its deflection indicates a proportional concentration amount of antigen-antibody interaction. The Valproic acid drug concentration of 50~500 μg/mL has been demonstrated by using this microcantilever biosensor with manipulating electric field.
Subjects
piezoresistive
microcantilever
protein
electric field
valproic acid
Type
thesis
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