The Effect of miR-17-92 cluster on Tumor Migration in Human Oral Squamous Cell Carcinoma
Date Issued
2011
Date
2011
Author(s)
Yang, Yu-Jen
Abstract
Metastasis is always an important concern in oral squamous cell carcinoma (OSCC) treatment, and migration is the first and crucial step in this process. To investigate this issue, we established a more aggressive cell line-TW2.6 MS-10 by transwell selection from low-migration ability oral cancer cell line: TW2.6. To better understand the effect of microRNAs (miRNA) profile in OSCC metastasis, the miRNA microarray analysis between TW2.6 and TW2.6 MS-10 was performed. According to the array data, a miRNA cluster miR-17-92, including miR-17, miR19b, miR-20a, miR-92a, was significantly down-regulated in TW2.6 MS-10 compare to the parental cells. To further confirm whether this cluster directly regulates the migration ability in OSCC cells, miR-17-92 cluster was over-expressed in TW2.6 MS-10 and SAS cells. The results showed that the migration abilities were remarkably suppressed compared to control vector clones (P < 0.01, P < 0.01, respectively). In order to figure out which miRNA(s) in this cluster is the dominant regulator(s) in OSCC migration instruction, we cloned miR17, miR19b, miR-20a and miR92 separately and found that miR-17 and miR-20a of miR-17-92 cluster seemed to play a key role in this migration regulation (P < 0.01).The clinical data also supported this finding. Cross matching by TargetScan and Microcosm to predict the suspicious downstream target(s) of miR-17 and mir-20a in this system, a possible downstream target ITGβ8 was found. Taken together, here we first defined a new pathological role of miR-17-92 cluster as a tumor suppressor miRNA in OSCC migration regulation machinery.
Subjects
cancer migration
miR-17-92 cluster
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-100-R98450017-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):681713e7b9711d75875a441ef044bbae