CD8+ canine peripheral blood lymphocytes and lymphokine-activated killer cells contain NK activity and NKp30 mRNA
Date Issued
2007
Date
2007
Author(s)
Lin, Yi-Chun
DOI
zh-TW
Abstract
Natural Killer (NK) cells are important effectors of the innate immune response. The function of NK cells is regulated by a balance between signals transmitted by activating and inhibitory receptors. Many types of inhibitory and activating NK cell receptors in the mammals have been identified, such as KIR, NCRs, ILT/ILR, 2B4, CD94/NKG2, NKG2D, Ly49, NKR-P1, and others. As for canine species, very little information is available on the inhibitory or activating NK receptors responsible for triggering natural cytotoxicity. In this study, we have identified and sequenced the full length of canine NKp30 mRNA from dog PBL and LAK. We have also discovered a subset of canine CD8+ cells from PBL and LAK that expressed CD3 and TCR a/b, containing higher NKp30 and other NK cell-related receptors mRNA than CD8- cells, including NKp44, NKG2D, 2B4, CD16 and CD56. This subset showed significantly higher NK activity comparing to CD8- cells. This subset has never been reported in other mammals, including human. It is a unique subset of cells in dogs. This finding is useful for the study on canine NK cell receptors.
Subjects
犬自然殺手細胞
周邊血液淋巴球
淋巴激素活化殺手細胞
自然毒殺型接受器3 (NKp30, NCR3)
CD8
自然毒殺作用
canine natural killer cells
peripheral blood lymphocytes (PBL)
lymphokine - activated killers (LAK)
natural cytotoxicity receptor 3 (NCR3, NKp30)
natural cytotoxicity
Type
thesis
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