Evaluation of anti-photoaging activity of hops extract on human skin cells
Date Issued
2015
Date
2015
Author(s)
Yang, Yi-Chen
Abstract
Skin aging can be divided into natural aging and photoaging, influenced by several factors. UV is considered one of the most important causes of skin photoaging, including wrinkling and hyperpigmentation. Wrinkle formation is relevant to degradation of extracellular matrix (ECM), especially collagen, the major materials that provides structural to the skin. UVA radiation promotes the generation of oxygen species (ROS) and synthesis of matrix metalloproteinases (MMPs) in dermal fibroblast. It was suggested that MMPs contribute to the breakdown of dermal interstitial collagen and other connective tissue components. Except direct damage in skin cells, UVB may accelerate melanogenesis and cause DNA or protein damage, and even lead to cancer. In previous studies, hops have attracted a great deal of attention as a source of small molecules such as humulone, lupulone and xanthohumol with potential for beneficial effects on anti-bacterial, anti-inflammatory, anti-oxidation and anti-cancer. Hops bitter acid and xanthohumol can inhibit MMP-1 and MMP-8 activity in vitro. Xanthohumol can stimulate elastin and type I collagen production in fibroblasts. The aim of this study was to evaluate pretreatment or co-treatment of HWE and HEE with UVA or UVB for skin anti-photoaging potential, UVA and UVB were respectively induced CCD-966SK and HaCaT cells, leading to acute light damage. The results showed that, the inhibition rate of HWE and HEE to mushroom tyrosinase were very limited. Thought HEE has higher antioxidant potential, it may be phototoxic. In UV-induced model, HWE can significantly enhance cell viability, while reducing the ROS production in CCD-966SK and HaCaT cells. Moreover, HWE inhibited the secretion of MMP-1, and we found that 100 μg/mL HWE can increase collagen production in CCD-966SK cells. At high doses of UVB irradiation, both treatments can suppress DNA damage, showing that HWE has anti-photoaging effect. In conclusion, these results show that HWE is more suitable than HEE for skin care products ingredients.
Subjects
hops extract
skin photoaging
matrix metalloproteinases
collagen
tyrosinase
SDGs
Type
thesis