Increased PD-1 and decreased CD28 expression in chronic hepatitis B patients with advanced hepatocellular carcinoma
Journal
Liver International
Journal Volume
30
Journal Issue
9
Pages
1379-1386
Date Issued
2010
Author(s)
Abstract
Background/aims: Hepatitis B infection is a well-known cause of hepatocellular carcinoma (HCC). This study aims to investigate the role that the co-stimulatory molecule CD28 and co-inhibitory molecule programmed death-1 (PD-1) play in compromising the function of tumour-infiltrating lymphocytes (TIL) in hepatitis B virus (HBV)-related HCC. Methods: A total of 45 patients with HBV-related HCC were enrolled during the period February 2008 to March 2010. The immune phenotype and the expression of PD-1, CD28 and CD127 in TIL in biopsy specimens and in peripheral blood lymphocytes (PBL) from the same patients were analysed by flow cytometry. Results: Among the 45 patients, there was a male predominance (80%) and the mean age was 50 ± 13.68 years (range: 29-71). The majority of TIL were CD45RO+CD69+. PD-1 expression was higher and CD28 and CD127 expression levels were lower in TIL than in PBL. The prevalence of portal vein thrombosis was 40%. Furthermore, tumour thrombosis invasion into the portal vein correlated with the expression level of the PD-1 co-inhibitory molecule. Conclusion: PD-1+ tumour-infiltrating lymphocytes correlate with portal vein thrombosis and might serve as a potential prognostic marker of and a novel therapeutic target for HBV-related HCC. ? 2010 John Wiley & Sons A/S.
Subjects
CD28; Hepatocellular carcinoma; PD-1; Tumour-infiltrating lymphocytes
SDGs
Other Subjects
CD28 antigen; interleukin 7 receptor; programmed death 1 receptor; adult; aged; antigen expression; article; cell function; cell isolation; controlled study; female; flow cytometry; hepatitis B; Hepatitis B virus; human; human cell; liver cell carcinoma; major clinical study; male; memory T lymphocyte; peripheral lymphocyte; phenotype; portal vein thrombosis; protein expression; tumor thrombus; Adult; Aged; Antigens, CD; Antigens, CD28; Apoptosis Regulatory Proteins; Carcinoma, Hepatocellular; Female; Hepatitis B, Chronic; Humans; Liver Neoplasms; Lymphocytes, Tumor-Infiltrating; Male; Middle Aged; Neoplasm Staging; Prognosis
Type
journal article