The effect of lotus leaf methanolic extract on obese rat
Date Issued
2007
Date
2007
Author(s)
Chiu, Yung-Fang
DOI
zh-TW
Abstract
Obesity has been a universal concern because it can be the cause of a lot of disease. The aim of our research is to estimate the effect of lotus leaf which is often used as diet tea in Chinese herbs on reducing body fat.
According to the Compendium of Materia Medica, a classic book of Chinese herbs, lotus leaf has a curing effect on obesity. In our experiment, we divided 60 SD rats into two groups, control and experiment group. In the control group we fed the rats with Purina 5001 commercial diet (3.4kcal/g). In the experiment group we fed the rats with high-energy (HE) diet (4.5kcal/g). The methanolic extract of lotus leaf（LLM）was added into high-energy diet to feed the experiment group. We used different dosages which were 0%, 0.45%, 1.59%, 4.03% and 5.38%. Before feeding the high-energy diet, we made the experiment group body weight 20% more than the control group (p<0.05). After that, we started to feed the experiment group with LLM for 4 weeks and then sacrificed the rats.
We found that feeding diet with LLM did not influence the amount of ingestion in SD rats. However, we found a significant decrease of body weight (p<0.01) in rats that were fed with LLM at dosage of 4.03%, 5.38% compared with those fed with HE diet. There was a significant decrease of serum TG in rats fed with 1.59%, 4.03%, and 5.38% LLM compared with HE diet. We also found a significant increase of serum TC in 1.59% and 5.38% LLM groups compared with HE diet.
In addition, in groups of feeding 1.59% and 5.38% LLM, HDL significantly increased compared with HE group. We also found that the concentration of LLM was negatively correlated with the decrease of blood sugar.
In the results of liver TG, there were significant different (p<0.05) in groups of 4.03% and 5.38% LLM compared with HE group. The liver TC decreased in groups of 0.45%, 1.59%, 4.03%, and 5.38% LLM.
In the results of fecal TG, there were significant different (p<0.05) in 4.03% and 5.38% LLM groups compared with HE group. We also found that in fecal TC, there was an increase of body cholesterol elimination in groups of 1.59%, 4.03% and 5.38% LLM.
Finally, we observed the diameter of adipose cells and found smaller diameter in high dosage group (4.03% and 5.38%) than in HE group (p<0.05).
Key Word： obesity、lotus leaf methanolic extract (LLM)、SD rat、adipose cell
Subjects
肥胖
荷葉甲醇萃出物
SD大白鼠
脂肪細胞
obesity
lotus leaf methanolic extract
SD rat
adipose cell
SDGs
Type
thesis
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