Chitosan biotinylation and electrodeposition for selective protein assembly
Journal
Macromolecular Bioscience
Journal Volume
8
Journal Issue
5
Pages
451-457
Date Issued
2008
Author(s)
Shi, X.-W.
Liu, Y.
Lew
owski, A.T.
Wu, L.-Q.
Wu, H.-C.
Ghodssi, R.
Rubloff, G.W.
Bentley, W.E.
Payne, G.F.
Abstract
An alternative route to protein assembly at surfaces based on using the unique capabilities of biological materials for the spatially selective assembly of proteins is described. Specifically, the stimuli-responsive properties of aminopolysaccharide chitosan are combined with the molecular-recognition capabilities of biotin-streptavidin binding. Biotinylated chitosan retains its stimuli-responsive properties and is capable of electrodepositing at specific electrode addresses. Once deposited, it is capable of binding streptavidin, which can mediate the subsequent assembly of biotinylated proteins. Spatially selective protein assembly using biotinylated Protein A and fluorescently-labeled antibodies is demonstrated.
Type
journal article