Functional Analysis of Adipokinetic Hormone Signaling in Bombyx mori
Journal
Cells
Journal Volume
9
Journal Issue
12
Start Page
2667
ISSN
2073-4409
Date Issued
2020-12-11
Author(s)
Sehadova, Hana
Takasu, Yoko
Zaloudikova, Anna
Sauman, Ivo
Sezutsu, Hideki
Rouhova, Lenka
Kodrik, Dalibor
Zurovec, Michal
Abstract
Insect adipokinetic hormones (AKHs) are short peptides produced in the corpora cardiaca and are responsible for mobilizing energy stores from the fat body to the hemolymph. Three related peptides, AKH1, AKH2, and AKH/corazonin-related peptide (ACP) as well as three AKH receptors have been reported in Bombyx mori. AKH1 and AKH2 are specific for the AKHR1 receptor, whereas ACP interacts with the other two AKHRs. To assess the effect of the two silkworm AKHs and ACP in the regulation of energy homeostasis we examined the expression pattern of the three peptides and their receptors as well as their effect on the level of carbohydrates and lipids in the hemolymph. Our results support the hypothesis that only AKH1 and AKH2 peptides together with the AKHR1 receptor are involved in the maintenance of energy homeostasis. Because Bombyx AKHR1 (BmAKHR1) seems to be a true AKHR we generated its mutation. The BmAKHR1 mutant larvae display significantly lower carbohydrate and lipid levels in the hemolymph and reduced sensitivity to starvation. Our study clarifies the role of BmAKHR1 in energy homeostasis.
Subjects
BMSK0010951
Bommo-AKH1
Bommo-AKH2
NM_001043584
Silkworm
TALEN
Targeted mutagenesis
SDGs
Publisher
MDPI AG
Description
Article number 2667
Type
journal article