Preparation and evaluation of β-TCP powder and crosslinked gelatin composite as bone substitute
Resource
CHINESE JOURNAL OF MEDICAL AND BIOLOGICAL ENGINEERING v.14 n.1 pp.47- 52
Journal
CHINESE JOURNAL OF MEDICAL AND BIOLOGICAL ENGINEERING
Journal Volume
v.14
Journal Issue
52
Pages
-
Date Issued
1994
Date
1994
Author(s)
Abstract
A mixture of β-TCP with formaldehyde crosslinked gelatin (GTF) was developed as a bone substitute in this study. The content of formaldehyde in the composite was used to control physical and biological properties of the gelatin structure which were in term of crosslinking gelatin molecules, solubility and biodegradation of the reconstituted matrices. Formaldehyde, however, has been reported a potential toxic substance which would cause inflammation and severe tissue response. The purpose of this study was to evaluate the toxicity of the developing material after soaked in a normal saline for a period of time. The residual formaldehyde was extracted from the composite by soaking the GTF in a normal saline for 1,2 ,4,8 days. The extracted solutions were added into the cell culture mediums with a ratio of 1:4. The cell number and cell morphology were calculated and observed to evaluate the GTF cytotoxic effect upon rat myoblasts, respectively. The rabbit femoral condyle test and rat subcutaneous implant were also described in the study. The histological examinatdion and microangiography were used to evaluate the bone regeneration and revascularization of the GTF in in vivo environment.#0557 #