Generation and characterization of monoclonal antibodies against dengue virus type 1 for epitope mapping and serological detection by epitope-based peptide antigens
Journal
Clinical and Vaccine Immunology
Journal Volume
14
Journal Issue
4
Pages
404-411
Date Issued
2007
Author(s)
Abstract
Dengue virus (DEN), the pathogen behind dengue hemorrhagic fever, remains a public health problem in Asia and South America. In this study, monoclonal antibodies (MAbs) against DEN serotype 1 (DEN-1) were generated by fusing NSI/1-Ag4-1 mouse myeloma cells with lymphocytes from BALB/c mice immunized with DEN-1. Twelve MAbs were found to react specifically to the DENs by enzyme-linked immunosorbent assay, immunofluorescence analysis, and immunoblotting analysis. Five MAbs, namely, DA4-7, DA6-7, DA9-5, DA10-2, and DA11-13, were found to react with envelope proteins of DEN-1. Two serotype-specific MAbs of DEN-1, DA6-7 and DA11-13, were further shown to neutralize DEN-1 infection by a plaque reduction neutralization test. The neutralizing epitopes of these MAbs were further identified from a random peptide library displayed on phage. Immunopositive phage clones reacted specifically with these MAbs and did not react with normal mouse serum. Epitope-based peptide antigens were proved able to detect antibodies in serum samples collected from DEN-1-infected patients but not in those taken from DEN-2-infected patients or healthy controls. We believe that these MAbs and neutralizing epitopes will provide information that will lead to the development of DEN-1 serotype-specific diagnostic reagents and vaccines. Copyright ? 2007, American Society for Microbiology. All Rights Reserved.
SDGs
Other Subjects
antigen; monoclonal antibody; monoclonal antibody da10 2; monoclonal antibody da11 13; monoclonal antibody da4 7; monoclonal antibody da6 7; monoclonal antibody da9 5; unclassified drug; animal cell; animal experiment; article; controlled study; dengue; Dengue virus; drug mechanism; epitope mapping; female; human; mouse; nonhuman; pathogenesis; priority journal; serology; virus strain; Animals; Antibodies, Monoclonal; Antigens, Viral; Cell Line; Dengue Virus; Epitope Mapping; Humans; Mice; Peptides
Type
journal article