Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines
Journal
Research in Veterinary Science
Journal Volume
88
Journal Issue
2
Pages
285-293
Date Issued
2010
Author(s)
Abstract
Three canine mammary tumor (CMT) cell lines, namely DE-E, DE-F and DE-SF, have been established from a surgically excised specimen of a malignant mammary tumor. These CMT cell lines have been cultured for over 200 passages. The cell doubling time was estimated to be approximately 30 h for all three cell lines. DE-E, DE-F and DE-SF were epithelial, fibroblast and spindle fibroblast in morphology, respectively. Under electron microscope, DE-F and DE-SF cells displayed a higher nucleus/cytoplasm ratio as compared with DE-E. Variation in chromosome number was also observed in the three cell lines. In addition to the morphological characteristics, these cell lines displayed differential patterns of several known mammary tumor cell markers. Following xenotransplantation of the CMT cells into nude mice, DE-F and DE-SF developed tumors within 2 weeks, whereas DE-E failed to develop any visible tumor up to 8 weeks after injection. Lastly, the CMT cell lines exhibited differential chemoresistance to several anti-tumor drugs, including melatonin, cyclosporine A, tamoxifen and indole, suggesting that these cell lines can be used as a comparative experimental model for the tumorigenesis of mammary carcinomas and a valuable tool for anti-cancer drug screening. ? 2009 Elsevier Ltd. All rights reserved.
Subjects
Anti-tumor drugs; Canine; Mammary tumor; Tumorigenesis
SDGs
Other Subjects
antineoplastic agent; cyclosporin A; cytokeratin 14; cytokeratin 17; cytokeratin 18; cytokeratin 19; cytokeratin AE1; cytokeratin AE3; doxorubicin; estrogen receptor; etoposide; indole; melatonin; progesterone receptor; protein Bax; protein bcl 2; protein p53; protein p63; resveratrol; retinamide; smooth muscle actin; tamoxifen; unclassified drug; vimentin; animal cell; animal experiment; article; breast tumor; cancer cell culture; cancer resistance; cancer transplantation; carcinogenesis; cell structure; chromosome number; controlled study; dog; drug screening; drug sensitivity; electron microscopy; mouse; nonhuman; nude mouse; protein expression; Animals; Antineoplastic Agents; Cell Line, Tumor; Dog Diseases; Dogs; Female; Gene Expression Regulation, Neoplastic; Mammary Neoplasms, Animal; Mice; Mice, Nude; Neoplasm Proteins; Neoplasms, Experimental; Tumor Markers, Biological; Mus musculus
Type
journal article