Study on eggshell membranes collagen extraction and functional properties of hydrolysates
Date Issued
2005
Date
2005
Author(s)
Wen, Chi-Ju
DOI
zh-TW
Abstract
Liquid egg factory had produced a lot of eggshell. In fact, collagen type Ⅰ, Ⅴ and Ⅹ have been found in the eggshell membranes ( Wong et al., 1984 ). Collagen is a composed of tropocollagen monomers arranged in overlapping fibrils that are configurated in three non-identical coiled peptide chains. Collagen and associated hydrolysate have desirable characteristics for various industrial applications. For example, they are not hazardous to human health, are capable of water retention on the molecular scale, are essential biological nutrients as can serve as chromatographic carrier particles. They can be used in materials associated with or included in cosmetics, artificial organs, etc. This study had two purposes, one was to establish a process to separate eggshell membranes from eggshell and to extract the collagen from eggshell membranes. Another one was to study the eggshell membranes collagen functional properties in order to increase eggshell membrane’s commercial application.
Eggshell are cracked and then washed with water in order to separate eggshell membranes from eggshell and use acetate acid to extracted collagen from eggshell membranes. The yield ratio of collagen extracted from eggshell membranes was 14.28%.
In functional test, the collagen was hydrolyzed for 1 - 5 h by four different enzymes of alcalase, neutrase, protease, trypsin. We measure the antioxidative activity and angiotensin converting enzyme (ACE) inhibitory activity of each hydrolysates at different temperature and pH. The results showed that among the four enzymes the sample which hydrolysates digested with alcalase and protease had expressed well on antioxidant ability and reducing ability. The hydrolysates digested with trypsin show the higher scavenging DPPH free radical ability than other enzymes. The Chelating ferrous ion ability and scavenging superoxide anion ability expressed well when hydrolyzed by neutrase. The hydrolysates digested with trypsin revealed a highest ACE inhibitory activity. The antioxidative activity and angiotensin converting enzyme inhibitory activity could be futher enhanced by hydrolysis time (1 - 5 h), hydrolysis temperature (50、60、70℃) and peptide concentraction. However, pH did not affect the antioxidative activity and angiotensin converting enzyme inhibitory activity of the hydrolysates.
Subjects
蛋殼膜
膠原蛋白
水解
抗氧化
抑制ACE
eggshell emebrane
collagen
hydrolysis
antioxidant
ACE inhibitory
SDGs
Type
thesis
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