Transcriptional Analysis of the Ribonucleotide Reductase Genes of Shrimp White Spot Syndrome Virus
Resource
Virology 277 (1): 92-99
Journal
Virology
Pages
92-99
Date Issued
2000
Date
2000
Author(s)
Tsai, Meng-Feng
Lo, Chu-Fang
Mari?lle C. W. van Hulten
Tzeng, Huey-Fen
Chou, Chih-Ming
Huang, Chang-Jen
Wang, Chung-Hsiung
Lin, Jung-Yaw
Vlakand, Just M.
Kou, Guang-Hsiung
Abstract
The causative agent of white spot syndrome (WSS) is a large double-stranded DNA virus, WSSV, which is probably a representative of a new genus, provisionally called Whispovirus. From previously constructed WSSV genomic libraries of a Taiwan WSSV isolate, clones with open reading frames (ORFs) that encode proteins with significant homology to the class I ribonucleotide reductase large (RR1) and small (RR2) subunits were identified. WSSV rr1 and rr2 potentially encode 848 and 413 amino acids, respectively. RNA was isolated from WSSV-infected shrimp at different times after infection and Northern blot analysis with rr1- and rr2-specific riboprobes found major transcripts of 2.8 and 1.4 kb, respectively. 5' RACE showed that the major rr1 transcript started at a position of -84 (C) relative to the ATG translational start, while transcription of the rr2 gene started at nucleotide residue -68 (T). A consensus motif containing the transcriptional start sites for rr1 and rr2 was observed (TCAc/tTC). Northern blotting and RT-PCR showed that the transcription of rr1 and rr2 started 4-6 h after infection and continued for at least 60 h. The rr1 and rr2 genes thus appear to be WSSV "early genes."
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