Structural characterization and transcriptional pattern of two types of carp rhodopsin gene
Resource
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 125 (1): 37-45
Journal
Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology
Pages
37-45
Date Issued
2000
Date
2000
Author(s)
Su, Chih-Ying
Lim, Jormay
Tsai, Huai-Jen
Abstract
This work characterizes the genomic structures of two types of carp (Cyprinus carpio) rhodopsin (cRh) gene, i.e. type I (cRh-I) and type II (cRh-II). Two types of cRh gene share only 45.6% polynucleotide identity in the upstream region from nucleotide -3436 to +97. However, three conserved regions are found. Homologies to the consensus recognition sites for transcription factors, Crx and Nrl, which are involved in photoreceptor-specific expression, are also observed in cRh genes. With specific polymerase chain reaction (PCR) primers, the two types of cRh gene can be clearly discriminated from each carp genome. Most carps exhibit both types of cRh gene, however, there are still carps possessing either cRh-I or cRh-II. Both cRh-I and cRh-II mRNAs are expressed at an approximately equal level in both eyes extracted from a carp carrying both types of cRh gene.
SDGs
File(s)![Thumbnail Image]()
Loading...
Name
07.pdf
Size
624.14 KB
Format
Adobe PDF
Checksum
(MD5):a55ab46d29cb665aa3fa67da27d5c2d7