Podocalyxin-like 1 promotes invadopodia formation and metastasis through activation of Rac1/Cdc42/cortactin signaling in breast cancer cells
Journal
Carcinogenesis
Journal Volume
35
Journal Issue
11
Pages
2425
Date Issued
2014-11-01
Author(s)
Lin, Cheng Wei
Sun, Min Siou
Liao, Mei Ying
Chung, Chu Hung
Chi, Yi Hsuan
Chiou, Li Tin
Yu, John
Wu, Han Chung
Abstract
Metastatic disease is the leading cause of cancer mortality. Identifying biomarkers and regulatory mechanisms is important toward developing diagnostic and therapeutic tools against metastatic cancer. In this study, we demonstrated that podocalyxin-like 1 (PODXL) is overexpressed in breast tumor cells and increased in lymph node metastatic cancer. Mechanistically, we found that the expression of PODXL was associated with cell motility and invasiveness. Suppression of PODXL in MDA-MB-231 cells reduced lamellipodia formation and focal adhesion kinase (FAK) and paxillin phosphorylation. PODXL knockdown reduced the formation of invadopodia, such as inhibiting the colocalization of F-actin with cortactin and suppressing phosphorylation of cortactin and neural Wiskott-Aldrich syndrome protein. Conversely, overexpression of PODXL in MCF7 cells induced F-actin/cortactin colocalization and enhanced invadopodia formation and activation. Invadopodia activity and tumor invasion in PODXL-knockdown cells are similar to that in cortactin-knockdown cells. We further found that the DTHL motif in PODXL is crucial for regulating cortactin phosphorylation and Rac1/Cdc42 activation. Inhibition of Rac1/Cdc42 impeded PODXL-mediated cortactin activation and FAK and paxillin phosphorylation. Moreover, inhibition of PODXL in MDA-MB-231 cells significantly suppressed tumor colonization in the lungs and distant metastases, similar to those in cortactin-knockdown cells. These findings show that overexpression of PODXL enhanced invadopodia formation and tumor metastasis by inducing Rac1/Cdc42/cortactin signaling network.
Subjects
GLOMERULAR EPITHELIAL-CELLS; MAJOR SIALOPROTEIN; N-WASP; TYROSINE PHOSPHORYLATION; MATRIX-METALLOPROTEINASE; ACTIN REORGANIZATION; PROSTATE-CANCER; ARP2/3 COMPLEX; STEM-CELLS; CORTACTIN
SDGs
Publisher
OXFORD UNIV PRESS
Type
journal article