DIFFERENCES IN REPLICATION CAPACITY BETWEEN ENTEROVIRUS 71 ISOLATES OBTAINED FROM PATIENTS WITH ENCEPHALITIS AND THOSE OBTAINED FROM PATIENTS WITH HERPANGINA IN TAIWAN
Resource
JOURNAL OF MEDICAL VIROLOGY v.79 n.1 pp.60-68
Journal
JOURNAL OF MEDICAL
Journal Volume
VIROLOGY
Journal Issue
n.1
Pages
60-68
Date Issued
2007
Date
2007
Author(s)
KUNG, CHIEN-MIN
KING, CHWAN-CHUEN
LEE, CHUN-NAN
HUANG, LI-MIN
Abstract
The cellular-tropism and biological characteristics of enterovirus 71 (EV71) isolates in Taiwan (TW) were studied. Growth curve experiments were conducted using cell lines that were possibly exhibited pathogenesis, and RT-PCR and sequencing tests were undertaken to amplify the 5' non- coding region (5'-NCR). The encephalitis isolate EV71 TW98 NTU2078 was PBMCtropic, temperature-resistant (Tr) at 40 degrees C, and easier to replicate in HTB-14 (astrocytoma) than the herpangina isolate EV71 TW98NTU 1186 (The viral yields were 100-fold higher than those of the herpangina isolate EV71 TW98NTU 1186 at 96 hr post infection.). The herpangina isolate EV71 TW98NTU 1186 was non-PBMC-tropic, and temperature-sensitive (Ts) at 40 degrees C. The replication of EV71 TW98NTU1186 in HTB-14 was lower. No EV71 isolate infected HTB-37 (human colon adenocarcinoma cells). The encephalitis EV71 isolate exhibited better replication and transmission in PBMCs and astrocytes than did the EV71 isolate without CNS involvement.
Subjects
enterovirus 71
virus variation
cellular tropism
Type
journal article
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