Processing bodies control the selective translation for optimal development of Arabidopsis young seedlings
Journal
Proceedings of the National Academy of Sciences of the United States of America
Journal Volume
116
Journal Issue
13
Pages
6451-6456
Date Issued
2019
Author(s)
Abstract
Germinated plant seeds buried in soil undergo skotomorphogenic development before emergence to reach the light environment. Young seedlings transitioning from dark to light undergo photomorphogenic development. During photomorphogenesis, light alters the transcriptome and enhances the translation of thousands of mRNAs during the dark-to-light transition in Arabidopsis young seedlings. About 1,500 of these mRNAs have comparable abundance before and after light treatment, which implies widespread translational repression in dark-grown seedlings. Processing bodies (p-bodies), the cytoplasmic granules found in diverse organisms, can balance the storage, degradation, and translation of mRNAs. However, the function of p-bodies in translation control remains largely unknown in plants. Here we found that an Arabidopsis mutant defective in p-body formation (Decapping 5; dcp5-1) showed reduced fitness under both dark and light conditions. Comparative transcriptome and translatome analyses of wild-type and dcp5-1 seedlings revealed that p-bodies can attenuate the premature translation of specific mRNAs in the dark, including those encoding enzymes for protochlorophyllide synthesis and PIN-LIKES3 for auxindependent apical hook opening. When the seedlings protrude from soil, light perception by photoreceptors triggers a reduced accumulation of p-bodies to release the translationally stalledmRNAs for active translation of mRNAs encoding proteins needed for photomorphogenesis. Our data support a key role for p-bodies in translation repression, an essentialmechanism for proper skotomorphogenesis and timely photomorphogenesis in seedlings. ? 2019 National Academy of Sciences. All Rights Reserved.
Subjects
Light
P-bodies
Photomorphogenesis
Skotomorphogenesis
Translation
SDGs
Type
journal article
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