Dietary phytoestrogen regulates estrogen receptor gene expression in human mammary carcinoma cells
Journal
Nutrition Research
Journal Volume
19
Journal Issue
10
Pages
1447-1457
Date Issued
1999
Author(s)
Abstract
The level of estrogen receptor mRNA and cell proliferation were examined in the estrogen responsive human breast carcinoma cell lines MCF-7 and T-47D upon the addition of biochanin A, a dietary phytoestrogen present in plant foods. Results show that T-47D cell growth was stimulated by biochanin A at low concentrations (<1.76x10-5M), but inhibited at higher concentration (>3.52x10-5 M). The elevated T-47D cell growth due to biochanin A treatment was effectively blocked by the addition of a pure antiestrogen ICI 182,780. A close association between cell proliferation and estrogen receptor mRNA was noted in MCF-7 and T-47D cells in response to biochanin A. The estrogen receptor mRNA increased at levels at cell growth stimulatory concentrations of biochanin A and decreased at growth inhibitory concentrations. Simultaneous addition of antiestrogen abolished the increase of estrogen receptor mRNA in response to biochanin A in T-47 D cells. Our results indicate that the effects of biochanin A on cell proliferation may be mediated through estrogen receptor in estrogen responsive human breast carcinoma cells. In addition, they demonstrate that plant secondary metabolites such as the dietary phytoestrogens exist in nature that may contribute to the total estrogen exposure of the human population.
Subjects
Biochanin A; Estrogen; Estrogen Receptor; Human breast carcinoma; Phytoestrogen
SDGs
Other Subjects
antiestrogen; biochanin A; estrogen receptor; fulvestrant; messenger RNA; phytoestrogen; article; breast carcinoma; cancer cell culture; cell growth; cell proliferation; cell strain MCF 7; controlled study; dietary intake; gene expression regulation; hormonal regulation; human; human cell; priority journal; Mink cell focus-forming virus
Type
journal article