Comparison of bacterial communities and their functional profiling using 16S rRNA gene sequencing between the inherent serpentine-associated sites, hyper-accumulator, downgradient agricultural farmlands, and distal non-serpentine soils
Journal
Journal of Hazardous Materials
Journal Volume
431
Date Issued
2022
Author(s)
Abstract
This study aims to determine and compare the bacterial community and functional profiles associated with serpentine sites, innate hyper-accumulating weed, downgradient agricultural farmlands and non-serpentine sites using 16S rRNA gene sequencing. Elemental analysis revealed that the serpentine rock and weathered soil have higher magnesium, nickel, chromium, magnesium/calcium and lower calcium/magnesium ratios and agricultural farmlands have recorded elevated chromium. Proteobacteria were found predominant, except the non-serpentine site which was rich in Cyanobacteria. PCA analysis at the genus level indicates the uniqueness of different experimental groups, except the hyperaccumulators which exhibited relatively less dissimilarity. The shift analysis showed the serpentine sites were characterized by the abundance of bacteria having heavy metal effluxion. The hyper-accumulating weeds were higher in plant growth-promoting bacteria expressing tolerance against heavy metals toxicity such as nickel, chromium, cobalt and arsenic. Besides, the agricultural lands were abundant in wetland-associated methanogens and metal (manganese, iron and zinc) transporting function related bacteria. The results suggest that the inherent edaphic factors including heavy metal content, the interacting behavior of hyperaccumulator's rhizosphere microbiota with soil and anthropogenic activities such as agricultural practices could be a major determinant of the variation in the bacterial community selection and abundance in the respective study sites. © 2022 Elsevier B.V.
Subjects
16S rRNA gene sequencing; Agriculture lands; Heavy metal resistance; Hyper-accumulator; Serpentine
Other Subjects
Chromium; Farms; Genes; Heavy metals; Magnesium; Metal analysis; Nickel; RNA; Serpentine; Soils; 16S rRNA gene sequencing; Agriculture land; Bacterial community; Downgradients; Heavy metal resistance; Hyper-accumulators; Nickel chromium; Proteobacteria; Serpentine soils; Weathered soils; Bacteria; arsenic; calcium; chromium; cobalt; iron; magnesium; nickel; RNA 16S; serpentine; zinc; comparative study; genetic analysis; heavy metal; microbial activity; microbial community; rhizosphere; serpentine; toxicity; agricultural land; Article; comparative study; controlled study; cyanobacterium; methanogen; microbial community; nonhuman; plant growth-promoting bacterium; principal component analysis; protein fingerprinting; Proteobacteria; rhizosphere; RNA sequencing; soil; wetland
Type
journal article