The Development of Bio-Sample Preparation Microfluidic Device
Date Issued
2014
Date
2014
Author(s)
Lin, Shiang-Chi
Abstract
Because of the needs of personalized healthcare, the development of microfluidic devices for miniaturized healthcare systems has been investigated intensively. Sample preparation is one of the important processes in the clinical diagnosis. It is crucial to ensure proper pre-treatment for chemical analysis in various biomedical applications. However, the current sample process is tedious, time consuming, and requires professional operation. Moreover, the clinical instrument is expensive and hard to integrate with the personalized health care system. Therefore, in this thesis, our goal is to develop sample preparation microfluidic devices which are utilized to personal health care system. First, flow cytometry is a well-developed instrument which is usually used in the current sample preparation and clinical diagnosis. However, the traditional flow cytometry is unable to achieve the requirements of the personal health care systems. Therefore, we developed a microfluidic flow cytometry which is characterized of the portability and low cost. Furthermore, the microfluidic flow cytometry is composed of single channel layer and single sheath-flow inlet which is able to eliminate the tedious flow interconnections and complicated operation. Besides the micro flow cytometry, we further develop a particle filtration microfluidic device which is able to be driven through the electricity and low power consumption. Based on this design, it is possible to integrate the sample preparation microfluidic device into the current mobile device. This developed particle filtration microfluidic device is realized through the combining effect of the travelling-wave electroosmosis and electroosmosis on the surface of particle. In the experiments, we successfully filtered out the HL-60 cells and human blood cells. Furthermore, we demonstrated a fluid pump chip integrated with driving circuit using the standard CMOS fabrication. Finally, to expand the sample preparation function based on the electrokinetic mechanisms, we further developed a particle separation microfluidic device with the characteristics of small volume, low-power consumption, and in-situ pump. This particle separation microfluidic device is able to separate the target particles from the test sample. We used the travelling-wave electroosmosis and the dielectrophoresis to achieve the particle separation of the particles in diameter of 1mm and 15um. In addition, we could reduce the size difference of particle separation through tuning applied frequency. In conclusion, this thesis successfully proved three designed concepts for sample preparation microfluidic devices. We expect that these designs provide new methods to develop the sample preparation function utilized in the point-of-care testing, and the mobile healthcare systems.
Subjects
microfluidic device
sample preparation
electrokinetic
electroosmosis
dielectrophoresis
point-of-care testing
mobile healthcare
SDGs
Type
thesis
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