Expression and Effects of Glycoprotein B7H3 in Oral Cancer Cells
Date Issued
2010
Date
2010
Author(s)
Ku, Ko-Li
Abstract
The incidence and mortality of oral cancer is the sixth in the worldwide, and the mortality is continuing rising each year. Once metastasis is occurred in oral cancer, the opportunity for patient to be cured becomes indistinct. Therefore, investigating of the mechanism of oral cancer metastasis is very important. There are some studies report the over-expression of glycoprotein B7H3 in many cancers. It was also indicated that the over-expression of B7H3 might be related with the progression, metastasis, and low survival rate of cancer. B7H3 is a membrane glycoprotein, it can be expressed in immune cells and many cancer cells. In this study, the expression of B7H3 in oral squamous cell carcinoma cell lines was investigated. Small interfering RNA (siRNA) was applied for inhibiting the expression of B7H3 and the changes of mobility, adhesion, and proliferation of cancer cells were also investigated.
In this study, we found that the expression of glycoprotein in Ca-9-22, SAS, CAL27, HSC3 oral cancer cells is more than that in S-G normal cells with significant difference. Identification and confirmation of glycoprotein B7H3 was performed by mass analysis and western blot analysis. For further investigating the function of B7H3 in cancer cells, siRNA was transfected into cancer cells in Ca9-22 and SAS to knockdown B7H3, and the changes of the ability of mobility, adhesion, and proliferation of cancer cells were observed. The results of wound healing assay showed that the migration ability of transfected cells were lower than that of control cancer cells without siRNA transfection. Furthermore, migration and invasion assay also showed that B7H3 knockdown could reduce the migration and invasion potentials of Ca9-22 and SAS cancer cells. It was also found that cell adhesion in fibronectin and laminin is decreased. Whether B7H3 was knockdown in cancer cells, there is no significant effect on adhesion to collagen Ⅳ (p > 0.05). Besides, the MTT data showed that B7H3 has no effect on cell proliferation. In conclusion, glycoprotein B7H3 plays an important role in oral cancer cells especially in cell migration and invasion. We suggest that B7H3 is a potential marker and might be useful for oral cancer therapy.
Subjects
oral cancer
glycoprotein
migration
invasion
adhesion
SDGs
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