Evaluation of enzyme-linked immunosorbent assay using recombinant 56-kDa type-specific antigens derived from multiple orientia tsutsugamushi strains for detection of scrub typhus infection
Journal
American Journal of Tropical Medicine and Hygiene
Journal Volume
100
Journal Issue
3
Pages
532-539
Date Issued
2019
Author(s)
Abstract
polymerase chain reaction-positive, scrub typhus patients, and 82 negative control serum samples from non-scrub typhus cases were used for evaluation of the recombinant TSA-based ELISA. We compared the performance of the mixed-TSA ELISA with immunofluorescence assay (IFA), which is considered the gold standard method for the serological diagnosis of scrub typhus. The results indicated that the sensitivity of IgM mixed-TSA ELISA (80.7%) was significantly higher than that of IgM IFA (68.8%). We demonstrated that the mixed-TSA ELISA had a high sensitivity and specificity and can be used for screening of scrub typhus patient in the early phase of the disease.
SDGs
Publisher
American Society of Tropical Medicine and Hygiene
Type
journal article