Calebin-A inhibits adipogenesis and hepatic steatosis in high-fat diet-induced obesity via activation of AMPK signaling
Journal
Molecular Nutrition and Food Research
Journal Volume
59
Journal Issue
10
Pages
1883-1895
Date Issued
2015
Author(s)
Abstract
Scope: Diet-induced obesity and associated nonalcoholic fatty liver disease have increased and become a major health problem worldwide. This study was conducted to investigate the chemopreventive effects of dietary Calebin-A, a curcuminoid, on differentiation of 3T3-L1 adipocytes and high-fat diet (HFD) induced obesity and hepatic steatosis. Potential mechanisms contributing to these effects were also elucidated. Methods and results: Calebin-A effectively and dose dependently suppressed accumulation of lipid droplets in adipocytes through the suppression of adipogenic specific factor peroxisome proliferator-activated receptor (PPAR) γ and fatty acid synthase and activated acetyl-CoA carboxylase. Dietary Calebin-A effectively decreased weight gain and relative perigonadal, retroperitoneal, and mesenteric fat weight in HFD-fed mice. Furthermore, Calebin-A markedly reduced hepatic steatosis and the serum levels of glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, total cholesterol, and triacylglycerol. These effects were associated with the downregulation of PPARγ, sterol regulatory element-binding protein-1, and particularly the activation of AMP-activated protein kinase α signaling found in both adipocytes and liver tissues. Conclusion: Taken together, these results demonstrated for the first time that Calebin-A suppressed adipocyte differentiation, prevented HFD-induced obesity, and improved hepatic steatosis, suggesting a novel application for the prevention and treatment of obesity and associated nonalcoholic fatty liver disease. ? 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Subjects
3T3-L1 adipocytes; Adipogenesis; AMP-activated protein kinase; Calebin-A; High-fat diet
SDGs
Other Subjects
calebin-A; cinnamic acid derivative; hydroxymethylglutaryl coenzyme A reductase kinase; terpene; 3T3 cell line; adipogenesis; adverse effects; animal; C57BL mouse; cell differentiation; drug effects; fatty liver; lipid diet; lipolysis; male; metabolism; mouse; obesity; pathology; physiology; signal transduction; 3T3-L1 Cells; Adipogenesis; AMP-Activated Protein Kinases; Animals; Cell Differentiation; Cinnamates; Diet, High-Fat; Fatty Liver; Lipolysis; Male; Mice; Mice, Inbred C57BL; Monoterpenes; Obesity; Signal Transduction
Type
journal article