Human T cell receptor (TCR) gene usage studies reveal (i) a third invariant TCR α gene and (ii) the molecular mechanism regulating the in vivo process of V-to-Jα rearrangement
Date Issued
2005
Date
2005
Author(s)
Li, Huei-Jhen
DOI
en-US
Abstract
T cells recognize antigens as peptides presented in the context of MHC class I or class II molecules with the fine specificity that is defined by the TCR. The majority of T cells express the TCR composed of disulfide-linked αβ heterodimer. Arstila et al. [1] estimated an average diversity of 4.5×105 different chains in the human naïve T cell repertoire. Regardless the calculated complexity, two invariant TCR α chains have been identified documented to be conserved among species. The first one is encoded by Vα24Jα18 and expressed on subset of NKT cells. The second one is encoded by Vα1Jα33. Here we report the finding of the third invariant TCR α chain, Vα1-Jα12 in humans.
The mechanisms regulating V-to-Jα recombination remain elusive. We found eight Jα segments, Jα1, 2, 14, 25, 51, 55, 59, and 60, that were not used in vivo in a cohort of 4,487 TCR α chain sequences. We call these Jαs as recombination cold spots. Histone acetylation, germline transcript and RSS were analyzed on these cold spots to understand the molecular mechanism regulating the in vivo process of V-to-Jα rearrangement.
Subjects
T細胞
接受器
不變T細胞接受器
α鏈
T cell receptor α chain
invariant TCR
Type
other
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