Numerical Study of Immunoassay Performance Enhancement of Biosensor with DC-Biased AC-Electrokinetic Effect
Date Issued
2012
Date
2012
Author(s)
Shen, You-Ru
Abstract
In the last two decades, microfluidic devices such as micromixers, micropumps, and biosensors are popularly investigated and developed. All list above are primarily employed for mixing, transporting, and detecting by means of externally or internally mechanical mechanisms. A biosensor has several advantages such as highly sensitive, tiny mass load detection and capable of monitoring dynamic biomolecular interaction in real time, to be used as the apparatus for the biomolecule detection.
AC electrokinetics is concerned with the study of flow stirring, movement and behavior of particles in suspension when they are subjected to AC electrical fields. When microfluidic devices of the microfabricated electrode systems are subjected to AC electrical fields, the AC electrokinetics involving the dielectrophoresis, AC electroosmosis, and ac electrothermal force will be generated according to charges of colloids, different properties of electrolytes or amplitude and frequency of external applied voltages.
The thesis presents a numerical study of DC-biased AC-electrokinetic (DC-biased ACEK) flow over a pair of symmetrical electrodes generated by a transverse conductivity gradient occurring at the electrodes when a DC-bias is applied, and use this mechanism on the biosensors. We use the finite element analysis software Comsol Multiphysics for DC-biased AC- electrokinetics applying on biosensors by means of several parameters charging to enhance the binding efficiency of immunoassay.
Subjects
immunoassay
biosensor
ACEK
DC-biased AC-electrokenetics
finite element method
Type
thesis
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