Submerged cultivation of Ganoderma lucidum in the medium containing leguminous plants and the anti-allergic effect of the fermentation products
Date Issued
2004
Date
2004
Author(s)
Wang, Hsing-Ling
DOI
zh-TW
Abstract
The objective of this research was to study the fermentation conditions for submerged cultivation of G. lucidum in a 5L fermenter using the medium containing leguminous plants. After the fermentation conditions were established the anti-allergic effects of the fermented products were investigated using cell culture systems. It was found that cultivation of G. lucidum in the medium consisting of 5% black soybean, 2% Astragalus membranaceus (Huangqi), and 2% glucose at 24℃, agitation speed 50 rpm, and aeration rate 0.5 vvm, yielded the highest crude triterpenes (31.82 mg/g D.W.). However, the fermentation conducted at 0.75 vvm and 18℃ yielded the highest amount of (1→3)-β-D-glucan. The maximum amount of aglycone was obtained on Day 5 during fermentation at an aeration rate 0.25 vvm and 0.75 vvm. The peptides contents increased during fermentation with time, and the content of total polyphenols reached a maximum value on Day 5, and decreased thereafter.
For the anti-allergic effect, the mononuclear cells (MNCs) from human and EL4 cell from mouse were used. Results indicated that the fermented products could reduce the secretion of TH1/TH2-type cytokine (IFN-γ、IL-2、IL-4) by the mitogen-activated MNCs and EL4 cells. And the fermented products obtained at 24℃ and aeration rates of 0.5 vvm and 0.75 vvm had the potential to become a health-promoting food ingredients with anti-allergic function.
Subjects
人類週邊血液單核細胞
抗過敏反應
(1→3)-β-D-glucan
異黃酮
粗三萜
靈芝
鼠科胸腺癌細胞
TH1/TH2細胞激素
黑豆
黃耆
Black soybean
HPBMNCs
Aglycone
Astragalus membranaceus (Huangqi)
Ganoderma lucidum
TH1/TH2-type cytokine
Anti-allergic
EL4 cell
Crude triterpenes
SDGs
Type
thesis
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