竹嵌紋病毒之分子生物學─子計畫三：竹嵌紋毒毒缺失RNA之研究(1/3)
Date Issued
1998
Date
1998
Author(s)
DOI
872311B002003B11
Abstract
In order to study the defective RNAs of bamboo mosaic virus (BaMV), we used RT-PCR
and TA-cloning methods to clone the defective RNAs from BaMV virions and obtained 35
cDNA clones. Nine cDNA clones were sequenced completely and proved to be the defective
RNAs of BaMV. According to sequence analysis, these defective RNAs were composed of
two noncontiguous regions, corresponding to 5' (474-868 nt) and 3' (252-382 nt) termini of
BaMV genome. Besides, three clones were found to contain a special fusion open reading
- 2 -
frame (ORF), which joined 5’ terminus of replicase gene with 3’ terminus of coat protein gene.
When assaying the biological activity of cloned defective RNAs, the RNA transcripts of
BaMV and defective RNAs were inoculated into tobacco protoplasts, incubated for 24 hours
and then analyzed by Northern blotting. The results indicated that at least six clones were
infectious and could be replicated by BaMV transcripts in protoplasts, but all lack of
interfering ability. The defective RNAs of clover yellow mosaic potexvirus (ClYMV) were
reported to contain a fusion ORF of replicase and coat protein genes, which was essential for
their infectivity. On the contrary, only D52 among the infectious defective RNAs of BaMV
maintained the similar fusion ORF. The maintenance of the reading frame of coat protein
gene was not required for the replication of defective RNAs of BaMV. From the above
results, the characters of defective RNAs of BaMV are different from those of ClYMV, and
the influence of fusion ORF on the defective RNAs of BaMV needs further study.
Subjects
Bamboo Mosaic
BaMV
Defective RNA
Publisher
臺北市：國立臺灣大學植物病理與微生物學系暨研究所
Type
other
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