Peripheral Blood PPARs Gene Expression, Metabolic Factors, and Liver Abnormalities in Chronic Hepatitis B
Date Issued
2014
Date
2014
Author(s)
Chen, Kuan-Wen
Abstract
Background: Studies have shown that peroxisome proliferator-activated receptors (PPARs) gene expression plays a critical role in lipid regulation, glucose metabolism and inflammation. The use of PPARs ligands was recently proved to be effective for treating non-alcoholic fatty liver disease. The aim of this study was to delineate the association between PPARs gene expression in peripheral blood mononuclear cells (PBMCs), metabolic factors and a spectrum of liver abnormalities among men with hepatitis B, who are at an extremely high risk of liver-related mortality.
Materials and Methods: The study subjects included a total of 372 male hepatitis B carriers, belonging to a cohort study of civil servants recruited in 1989-1992. History of diabetes mellitus (DM), body mass index (BMI) and blood transaminase were measured at study subjects’ enrollment into the cohort since 1989 and at follow-up and measurement of gene expression, while waist-to-hip ratio and ultrasonographic liver abnormalities were determined at follow-up and measurement of gene expression. We determined expression levels of PPARs in PBMCs by quantitative reverse transcription polymerase chain reaction. The median of each PPAR expression level was used as the cutoff point to categorize the subjects into “high” and “low” expression groups. Logistic regression was used to analyze the relationship between PPARs gene expression, metabolic factors, and liver abnormalities.
Results: Low PPARs gene expression were significantly associated with metabolic factors including overweight (BMI≧25 kg/m2), waist-hip ratio≧0.9 and prior DM. Furthermore, low expression of PPARα and PPARβ were significantly associated with liver cirrhosis detected by abdominal ultrasonography. After adjustment for age, cigarette smoking, alcohol consumption, DM, liver biochemical values, the odds ratio of liver cirrhosis was 2.995 (95% CI: 1.133-7.917) for low PPARα expression and 2.730 (95% CI: 1.010-7.379) for low PPARβ expression.
Conclusion: Low peripheral expression of PPARs that characterize DM and related conditions might be associated with advanced liver disease such as liver cirrhosis in hepatitis B carriers.
Subjects
B型肝炎
過氧化體增生活化受體PPARs
代謝因子
SDGs
Type
thesis
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