Development of Thermosensitive Polyn-Isopropylacrylamide-Co-2- Dimethylamino Ethyl Methacrylate-Based Nanoparticles for Controlled Drug Release
Resource
NANOTECHNOLOGY v.22 n.26 pp.AR 265608
Journal
NANOTECHNOLOGY
Journal Volume
v.22
Journal Issue
265608
Pages
-
Date Issued
2011
Date
2011
Author(s)
PENG, CHENG-LIANG
TSAI, HAN-MIN
YANG, SHU- JYUAN
LUO, TSAI-YUEH
LIN, WUU-JYH
SHIEH, MING- JIUM
SHIEH, MING-JIUM
Abstract
Thermosensitive nanoparticles based on polyN- isopropylacrylamide-co-2- dimethylaminoethylmethacrylate polyNIPA-co-DMAEMA copolymers were successfully fabricated by free radical polymerization. The lower critical solution temperature LCST of the synthesized nanoparticles was 41 degrees C and a temperature above which would cause the nanoparticles to undergo a volume phase transition from 140 to 100 nm, which could result in the expulsion of encapsulated drugs. Therefore, we used the polyNIPA-co- DMAEMA nanoparticles as a carrier for the controlled release of a hydrophobic anticancer agent, 7-ethyl-10-hydroxy- camptothecin SN-38. The encapsulation efficiency and loading content of SN-38-loaded nanoparticles at an SN-38/polyNIPA- co-DMAEMA ratio of 1/10 D/P = 1/10 were about 80% and 6.293% , respectively. Moreover, the release profile of SN-38- loaded nanoparticles revealed that the release rate at 42 degrees C above LCST was higher than that at 37 degrees C below LCST, which demonstrated that the release of SN-38 could be controlled by increasing the temperature. The cytotoxicity of the SN-38-loaded polyNIPA-co-DMAEMA nanoparticles was investigated in human colon cancer cells HT-29 to compare with the treatment of an anticancer drug, Irinotecan R CPT-11. The antitumor efficacy evaluated in a C 26 murine colon tumor model showed that the SN- 38-loaded nanoparticles in combination with hyperthermia therapy efficiently suppressed tumor growth. The results indicate that these thermo-responsive nanoparticles are potential carriers for controlled drug delivery.
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