Effects of Angelica sinensis and Astragalus membranaceus polysaccharides on proliferation and differentiation of hematopoietic stem cells from umbilical cord blood
Date Issued
2008
Date
2008
Author(s)
Lee, Jia-Gua
Abstract
The aim of this research was to investigate the effects of Angelica sinensis (AS) and Astragalus membranaceus (AM) polysaccharides on proliferation and differentiation of hematopoietic stem cells (CD34+ cells) from umbilical cord blood. This research was divided into three parts. In the first part, we confirmed that polysaccharides was the major hematopoietic component in the AS water extract. The Angelica sinensis polysaccharides (ASPS) were fractionated into four fractions by anion-exchange chromatography, and F 2 fraction had the highest hematopoietic activity among four fractions. The F2 fraction could stimulate secretion of hematopoietic growth factor (HGF), such as GM-CSF and IL-3, and promote colony-forming unit of granulocyte-macrophage (CFU-GM) formation. The molecular weight of F 2 fraction was between 51.28 kDa and 8.40 kDa, and composed of arabinose, galactose and galacturonic acid. In part II, we used the decoction - Danggui Buxue Tang (DBT), which is composed of AS and AM with weight ratio 1：5. We compared the hematopoietic activity of DBT, AS and AM, and found that the combination of AS and AM had synergizing on hematopoiesis, and AS and AM polysaccharides were the active component in DBT. DBT induced all HGF that we detected, including GM-CSF, IL-3 and M-CSF. Compared to F2 fraction, DBT could stimulate more kinds of HGF production, and this is the probably reason why DBT had higher hematopoietic effect than F2. DBT not only promoted the formation of CFU-GM but also promoted Burst-forming unit erythrocyte (BFU-E) colony formation. In part III, we compared the protective effect of F2 fraction and DBT against the damage caused by the chemotherapy drug Adriblastina. It was found that both the F 2 fraction and DBT rescued CD34+ cells viability and protected the colony formation ability of CD34+ cells. In this damage model, DBT was found to have better effects than F 2 fraction.
Subjects
Angelica sinensis
mononuclear cells-conditioned medium (MNC-CM)
hematopoietic growth factor (HGF)
umbilical cord blood
polysaccharide
hematopoiesis
colony-forming assay
Type
thesis
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