Regulation of inducible nitric oxide synthase by dietary phytoestrogen in MCF-7 human mammary cancer cells
Journal
Reproduction Nutrition Development
Journal Volume
40
Journal Issue
1
Pages
11-18
Date Issued
2000
Author(s)
Abstract
We examined the effects of the phytoestrogen biochanin A on the growth of the MCF-7 human breast cancer cell line. The results showed that biochanin A treatment induced dose- and time-dependent inhibition on MCF-7 cell growth at concentrations above 20 μg.mL-1. An examination of treated MCF-7 cell morphology revealed condensation of the chromosome and dehydration of the cytoplasm, suggesting apoptosis as an important factor in biochanin A-related cell growth inhibition. The results also showed that at a concentration of 40 μg.mL-1, biochanin A decreased the levels of inducible nitric oxide synthase, thus inhibiting the production of nitric oxide, a known second messenger and inducer of apoptosis, and affecting the overall cell protein pattern. No significant difference in superoxide dismutase protein levels were, however detected at concentrations of 40 or 100 μg.mL-1 of biochanin A. The data suggest that the inhibitory effects of biochanin A on human breast cancer cell growth are linked to inducible nitric oxide synthase and the associated production of nitric oxide.
Subjects
Nitric oxide synthase; Phytoestrogen; Proliferation; Superoxide dismutase
SDGs
Other Subjects
biochanin A; interferon; nitric oxide; nitric oxide synthase; phytoestrogen; superoxide dismutase; tamoxifen; article; breast cancer; cancer inhibition; cell growth; cell strain MCF 7; chromosome condensation; controlled study; endocrine disease; enzyme regulation; human; human cell; infertility; intracellular fluid; liver dysfunction; Mink cell focus-forming virus
Type
journal article