早期發育階段豬胚表現其胚源性基因之時間的特異性(2/3)
Date Issued
2000
Date
2000
Author(s)
鄭登貴
DOI
892313B002049
Abstract
In a previous study, we demonstrated the
expression of transforming growth factor-a
(TGF-a) gene in the epithelium of porcine
oviductal at a time corresponding to 4-cell stage
(Chang et al., 2000). Here we report on the
pTIG-1 (porcine TGF-a induced gene-1) was
induced in the porcine four cell embryo within 3
hours of TGF-a treatment (20 ng/ml) using
mRNA differential display. By further RT-PCR
using these specific primers, we obtain the
pTIG-1 mRNA was absent in in vivo developed
early four cell embryos but was detected in
eight cell and 16-cell up to blastocyst stage
embryo in vivo. However, TGF-a treatment of
in vitro cultured early four cell embryos
resulted in the appearance of pTIG-1 transcripts.
In situ hybridization analysis demonstrated that
the pTIG-1 was expressed late four cell
embryos. Sequencing the full-length pTIG-1
cDNA clone identified one open reading frames
of 3280 bp which encode 500 amino acids using
cDNA library screen. Sequence computer
analysis display that the nucleotide sequence of
the pTIG-1 gene was homologous to the mouse
Mo25 (mMo25) (Miyamoto et al.,1993). The
predicated amino acid sequence revealed that
the pTIG-1 might encode a Ca2+ binding protein
like as mMo25. In this study, we further
investigated the role of TGF-a on early
embryogenesis using an in vitro culture system
and mRNA differential display in
pre-implantation embryos. Indeed, we identified
a candidate gene, pTIG-1, the expression of
which seemed to be modulated by TGF-a
Publisher
臺北市：國立臺灣大學動物科學技術學系暨研究所
Type
report
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