過氧化體增生接受子γ在葛雷夫氏症眼病變扮演角色之研究
Other Title
The study of role of PPARγ in Graves´ ophthalamopathy
Date Issued
2000
Date
2000
Author(s)
黃天祥
DOI
892314B002075
Abstract
Graves' Ophthalmopathy (GO), the most
frequent extrathyrodial manifestation of
Graves' disease, remains a pathogenetic enigma
and a therapeutic dilemma. Support for the
concept that GO is of autoimmune origin
comes from the associated histopathological
changes. There is an increased volume of the
extraocular muscles and orbital connective and
adipose tissues; the extraocular muscles are
edematous due to increased production of the
hydrophilic glycosaminoglycans (GAGs) in the
orbital tissue; a marked infiltration of
immunocompetent cells is detectable. Orbital
fibroblasts may contribute to perpetuate the
ongoing immune reaction in the orbit by
protecting infiltrating T cells from apoptosis.
Orbital fibroblasts include a subpopulation of
cells (preadipocytes), which, under particular
hormonal stimulation, differentiate into
adipocytes and may contribute to the increased
volume of retroorbital adipose tissue. In the
present study, we succeed to isolate fibroblasts
from minced explants of connective tissue
removed in the course of transantral orbital
decompression surgery for GO. Using
Western blotting and Electrophoretic gel
mobility shift assays, the protein level and the
DNA binding affinity on PPAR response
element (PPRE) of nuclear PPARg in the
cultured Graves' and normal orbital
fibroblasts are not changed significantly. But
the protein amount of signal transducer and
activator of transcription (STAT3) is
decreased in the cultured Graves' orbital
fibroblasts than in the normal orbital
fibroblasts.
Subjects
Graves' Ophthalmopathy
Orbital Fibroblast
Peroxisome
Proliferator Activated Receptor γ
Proliferator Activated Receptor γ
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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