Dengue hemorrhage in a mouse model
Journal
Annals of the New York Academy of Sciences
Journal Volume
1171
Journal Issue
SUPPL. 1
Date Issued
2009
Author(s)
Abstract
Here we summarized our findings in the mouse model of the events that lead to dengue hemorrhage. Immunocompetent mice inoculated intradermally with DENV-2 strain 16681 develop hemorrhage locally or systemically. The incidence and the severity of hemorrhage development are dependent on the size of viral inoculum. The hemorrhage mice exhibit severe thrombocytopenia, prolonged bleeding time, and increased numbers of circulating endothelial cells. In the hemorrhage tissues, there is endothelial damage accompanied by infiltrating macrophages that secret TNF-α. The endothelial cells express iNOS and peroxynitrite and undergo apoptosis, indicating RNS and ROS production may lead to cell death. By using mice deficient in iNOS and phox47 and apocynin, we demonstrated that RNS and ROS are important to hemorrhage development after infection by DENV. Our mouse model offers the opportunity to test potential dengue vaccines and therapeutics to treat dengue hemorrhage and to test hemorrhage induction potentials of dengue viral strains. ? 2009 New York Academy of Sciences.
Subjects
animal model; dengue virus; endothelial cells; hemorrhage; nitric oxide; reactive oxygen species; TNF-α
SDGs
Other Subjects
apocynin; dengue vaccine; inducible nitric oxide synthase; peroxynitrite; reactive nitrogen species; reactive oxygen metabolite; tumor necrosis factor alpha antibody; inducible nitric oxide synthase; tumor necrosis factor alpha; virus vaccine; animal experiment; animal model; apoptosis; bleeding; bleeding time; cell death; controlled study; cytokine release; dengue; disease severity; endothelium cell; human; immunocompetent cell; in vitro study; incidence; mouse; nonhuman; protein expression; review; thrombocytopenia; tumor associated leukocyte; virus replication; animal; article; biosynthesis; bites and stings; blood; C57BL mouse; dengue; disease model; genetics; immunology; innate immunity; mosquito; pathology; pathophysiology; virology; Animalia; Dengue virus; Mus; Animals; Apoptosis; Bites and Stings; Bleeding Time; Cell Death; Culicidae; Dengue Hemorrhagic Fever; Disease Models, Animal; Endothelial Cells; Immunity, Innate; Mice; Mice, Inbred C57BL; Nitric Oxide Synthase Type II; Thrombocytopenia; Tumor Necrosis Factor-alpha; Viral Vaccines
Type
book