Effect of Trehalose Supplementation on Production Performance and Antioxidant Capacity of Dairy Goats
Date Issued
2015
Date
2015
Author(s)
Chen, Yu-Jin
Abstract
Trehalose, a neutral disaccharide composed of two molecules of glucose linked by an α,α-1,1 glucosidic linkage. It is used widely, particularly as food additive, cosmetic ingredient, and antioxidant. Oxidative stress leads to aging and disease in animals and is caused by an imbalance between the production of reactive oxygen species (ROS) and antioxidant activity. In Taiwan, both dairy cows and goats will suffer from heat stress during the summer, and this condition will affect their health and milk production performance. So, we hypothesized that dietary trehalose supplementation might protect against oxidative stress in dairy goats, thereby resulting in milk with low lipid peroxide and high antioxidant content. Nine Alpine dairy goats housed separately in individual pens were divided into 3 groups, with each group receiving a different dietary treatment (control diet, 1% trehalose-supplemented diet, and 1% maltose-supplemented diet) following a 3×3 Latin square design. During the experiment period, we collected feces, refusals, urine, milk, blood, and rumen fluid samples. Then we analyzed nutrients digestibility, blood biochemical value, milk and milk fatty acid composition, rumen fluid volatile fatty acids (VFAs) and rumen protozoa number, and antioxidant content in blood, milk, and rumen fluid. There was no significant difference in dry matter intake, milk composition and production, blood biochemistry parameters, rumen fluid pH, VFAs concentrate and protozoa number among three groups. In milk fatty acids composition, the trehalose group has higher C18:2 and conjugated linoleic acid (CLA) contents than the control group (p<0.05). Antioxidant activities were estimated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, relative superoxide dismutase (SOD) activity and thiobarbituric acid reactive substances (TBARS) assay, respectively. The results show that the trehalose treatment has higher DPPH scavenging activity in milk than the maltose group (P<0.05) , but has no difference compared to the control group. Additionally, there is no difference on SOD or TBARS among the three groups. Maybe because of the temperature-humidity index and diet formula, the experimental conditions can’t lead to effective heat-stress for these dairy goats. Based on the current results, our research demonstrated that trehalose supplementation in diet of dairy goats will change the milk fatty acid composition to increase C18:2, and CLA content compared to the control group. Besides, trehalose treatment has greater goat milk DPPH scavenging activity than the maltose treatment. These results suggest that adding trahalose in the diet of dairy goats might have positive effects potentially.
Subjects
trehalose
maltose
oxidative stress
antioxidant capacity
dairy goats
Type
thesis
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