The regulation of visfatin on genes related to lipidetabolism in porcine adipocytes
Date Issued
2007
Date
2007
Author(s)
Deng, Su-Jiun
Abstract
Visfatin is a visceral fat-specific adipocytokine that function to mimic insulin actions, including regulation of plasma glucose and triacylglycerol concentrations and enhancement of glucose entry. To investigate the role of porcine visfatin in lipid metabolism, we first produce the adipose tissue recombinant porcine visfatin and used it as an immunogen for monoclonal antibody. In preadipocytes, the expression of lipoprotein lipase (LPL) and peroxisome proliferators activated receptor gamma (PPARγ) mRNA was increased by visfatin (100 ng/mL) or insulin (100 ng/mL) treatment for 8 days (p<0.05). Transcript to aP2 was promoted by 25-fold in the present of insulin treatment compared with control group (p<0.05), whereas visfatin treatment failed to increase an aP2 expression. Moreover, adipocyte differentiation was induced by insulin treatment after 8 days, whereas visfatin treatment exerted no such effect. In differentiated porcine adipocytes, treatment of insulin and visfatin for 24 hours increased FAS mRNA (p<0.05), but had no effect on sterol regulatory element binding proteins 1c (SREBP-1c) expression. Visfatin also upregulated interleukin-6（IL-6）expression in porcine adipocytes. n conclusion, results of study demonstrated that visfatin up-regulates LPL expression in preadipocytes and thereby may facilitate to lipid uptake. It also increases the expression of FAS in adipocytes, by which lipogenic activity may be enhanced. Interestingly, visfatin may also regulate IL-6 expression. Our data suggest that visfatin not only has similar lipogenic function as insulin, it also involved in regulating some cytokine in porcine adipocytes.
Subjects
visfatin
porcine
lipid metabolism
Type
thesis
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