肝細胞癌及非腫瘤肝組織肝浸潤T細胞的分析-著重在CD25的表現
Date Issued
2003
Date
2003
Author(s)
陳健弘
DOI
912314B002403
Abstract
Background/Aims: Tumor may induce local immunosuppression and make the
tumor-infiltrating lymphocytes (TILs) functionally inhibited and lose the ability to clonal
proliferation and exert their cytotoxicity to tumor cells. Therefore, we investigated the phenotypic
analysis of TILs in hepatocellular carcinoma (HCC) tissues. Methods: Lymphocytes were isolated
from paired HCC tissues (TILs) and the corresponding non-tumor liver tissues (non-tumor-liver
infiltrating lymphocytes, NILs) of 28 patients. These lymphocytes were subjected to flow cytometric
analysis. Results: TILs had higher CD3 + /CD56 + ratio than NILs. Around 70%-90% NILs or TILs
expressed the antigen-experienced or memory phenotypes of CD45RA - / CD45RO + / CD62L - .
Around 60%-70% CD4 + or CD8 + NILs and TILs expressed the activation markers CD69 and
HLA-DR. However, we found that CD25 is under-expressed in both the CD8 + NILs and TILs. In
addition, more CD4 + CD25 + regulatory T cells were detected in the TILs than in the NILs.
Conclusions: Most of infiltrating lymphocytes from the HCC tissues and the corresponding
non-tumor liver tissues were activated and expressed antigen-experienced phenotypes. However, the
CD25 was underexpressed in the CD8 + TILs and the CD4 + CD25 + regulatory T cells were increased
in the TILs. These factors might impair the antitumor immunity in HCCs.
Subjects
Tumor-infiltrating lymphocytes
liver-infiltrating lymphocytes
regulatory T cells
hepatocellular carcinoma
activation
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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