QTL mapping for traits related to salinity tolerance in rice
Date Issued
2014
Date
2014
Author(s)
Lee, Chuan
Abstract
Salinity is one of the major abiotic stresses that limited the growth of crops. Salinity stress can be divided into two phases: the osmotic stress happened rapidly when plant senses high level of salt in soil; the ionic stress usually occurs later when too much sodium ion is accumulated in the shoot. Rice is the most salt sensitive crop among cereals, we hope to identify QTLs that significantly contribute to salt tolerance in rice. In this study we used two sets of rice diversity accessions for association mapping and IR64/Nipponbare recombinanat inbred lines for linkage mapping. For phenotypic traits, we measured stomata density, seedling height, leaf area and injury score under normal and salt conditions, we expect the accessions with lower stomata density could help reducing the transpiration rate, therefore sodium uptake in leaves is further minimized. Our result indicates that stomata density has weak positive relationship with injury score, and has moderate correlation with relative seedling height change and the reduction of leaf area. This suggests that stomata density might be involved in osmotic tolerance rather than ionic tolerance. For linkage mapping study, we mapped the significant loci on chromosome 2 and 6 explained 6 and 12% of the phenotypic variation of stomata density, respectively; and in these regions, the accessions with Nipponbare allele tend to have lower stomata density. These results demonstrate the contribution of stomata density to salinity tolerance at rice seedling stage and identify the accessions with multiple tolerance mechanisms, which can be further used in genetic breeding of salt tolerant rice.
Subjects
水稻幼苗
鹽逆境
氣孔密度
鹽害指數
葉面積
株高
單一核苷酸多型性
關聯性定位
連鎖定位
滑動視窗
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-103-R01621106-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):654ad0e93b083f8476207cf4479b10e9