花生蔟葉病菌質體DNAK和DNAJ基因之選殖與分析(2/2)
Date Issued
2002
Date
2002
Author(s)
林長平
DOI
902313B002321
Abstract
The PCR-based strategy was used for cloning and analysis of dnaK gene of
phytoplasma associated with peanut witchs’ broom. For nested-PCR, two pairs of
nucleotide primer were designed based on the conserved regions of bacterial dnaK
genes. A 750 bp dnaK gene fragment was amplified as predicted and further used
for screening an EcoRI-digested genomic library of PNWB phytoplasma. Four open
reading frames were identified in the order of ORF1, grpE, dnaK and dnaJ .
Chromosomal arrangement of these genes in phytoplasmas is identical to Clostridium
acetobutylium and other bacteria phylogenic closed to phytoplasmas. It implies the
primitive bacterial molecular chapone HSP70 mechine exsists in phytoplasma and the
mechanism it may involve when phytoplasma exposed to stress could be similar to
other prokaryotes.
Subjects
Heat shock protein
Mollicutes
nested-PCR
Publisher
臺北市：國立臺灣大學植物病理與微生物學系暨研究所
Type
other
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